This project aims to uncover the cellular processes influencing neurodegenerative disease pathogenesis. Specifically, the role of cell-cell communication in propagating the pathological features of these diseases. This project focuses on Extracellular Vesicles (EVs), membranous parcels released from cells and involved in intercellular communication, as vectors for this propagation. In this project we investigate the protein cargo of these EVs using highly advanced proteomics including mass tagging techniques. Examination of the EV proteome allows us to elucidate whether the EVs contain damaged and misfolded proteins and how these are packaged into the vesicles. This project will allow a better understanding of how cellular communication facilitates the spread and progression of neurodegeneration and provide insight into therapeutic pathways that can be explored in the future.