This project aims to analyze the circulating miRNA pre-operation of the TKA and knee tissue mRNA post primary TKA. We will bridge between the data of bioinformatics analyses and clinical scenarios with the expectation providing precise predictors for prognosis of arthrofibrosis and biomarkers for prevention and drug discovery. This is prospective study in collaboration with TORU clinical research aiming to recruit 300 patients’ blood and knee synovial tissue for plasma miRNA and tissue mRNA analysis including miRNA and mRNA sequencing, RT-RNA expression and protein assay. Dr Tom Ward, Senior Lecturer at ANU School of Medicine and Psychology (SMP), Jack Gong, a SPM student and Yixuan Li, Master student in our Lab, are working on this prospective study, which will pave a new way for precise prognosis and drug discovery for arthrofibrosis after TKA.

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